Coding

Part:BBa_K1806001

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM15_AUC_TURKEY   (2015-09-16)
Revision as of 23:15, 19 September 2015 by TLOS (Talk | contribs)

Helicobacter pylori catalase

This part is the coding sequence for the Helicobacter pylori catalase, which effectively catalyses the breakdown of hydrogen peroxide. The breakdown of hydrogen peroxide, which is an exothermic process, will be utilized to provide heat to the medium to increase temperature to counteract a shift that decreases the temperature of the host environment.

Cloning

The part was ligated to the pSB1C3 cloning vector and the transformation was verified with Colony PCR. Afterwards, the isolates cut with EcoR1 and Pst1 were cutchecked. The functionality of the cloning of these parts can be seen in the gel images taken and placed below. The images on the right show the expected bands in the gel run.

6001-1.png 6001-2.png

6001-3.png 6001-4.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 852
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1523
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None