Composite

Part:BBa_K1675011

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-15)
Revision as of 18:46, 15 September 2015 by Registry (Talk | contribs)

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Bxb1 recombinase device

The Bxb1 integrase is a DNA recombinase, more precisely a member of serine recombinase family. Bxb1 could be divided to two parts, gp35 and gp47. The gp35 part could recognize specific sequences, called attB and attP, and then integrate, invert, or excise dsDNA depending on the orientation of recognition sequences. When it inverts DNA sequence, the attB and attP sequences are changed into attL and attR, as other DNA recombinases do. Another part called Bxb1 gp47 binds to integrase-DNA complex and this complex flips inverts DNA back into original sequence by regenerating attB and attP sequences. So the efficiency of inversion mediated by Bxb1 is half and half.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1099
    Illegal XhoI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1738
    Illegal NgoMIV site found at 1825
    Illegal AgeI site found at 875
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1933


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Categories
Parameters
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