RNA

Part:BBa_K1777000:Design

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-13)
Revision as of 17:04, 13 September 2015 by Jxl (Talk | contribs) (→‎Design Notes)


two mir-21 binding sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use overlap PCR to synthesize the mir-21 binding domain. In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together.

Source

Renilla luciferase ofthis part come from psiCHECK-2 Vector.

References