Part:BBa_K1138000

pAW42-gs (ugaiGEM2)

The plasmid pAW42-gs(ugaiGEM2)consists of the gs gene inserted into the backbone of pAW42-vector. The gs gene encodes for geraniol synthase enzyme. This vector has puramycin marker for selection in Methanogens and ampicilin marker for selection in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1553
  Illegal XbaI site found at 1883
  Illegal XbaI site found at 1892
  Illegal SpeI site found at 6597
  Illegal PstI site found at 2421
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1553
  Illegal SpeI site found at 6597
  Illegal PstI site found at 2421
  Illegal NotI site found at 2608
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1553
  Illegal BglII site found at 1870
  Illegal BamHI site found at 2402
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1553
  Illegal XbaI site found at 1883
  Illegal XbaI site found at 1892
  Illegal SpeI site found at 6597
  Illegal PstI site found at 2421
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1553
  Illegal XbaI site found at 1883
  Illegal XbaI site found at 1892
  Illegal SpeI site found at 6597
  Illegal PstI site found at 2421
  Illegal AgeI site found at 538
  Illegal AgeI site found at 2444
  Illegal AgeI site found at 2620
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 4778
  Illegal BsaI.rc site found at 3161
  Illegal BsaI.rc site found at 3490
  Illegal SapI site found at 6442