DNA
Part:BBa_K1648005
Designed by: Leung King Pong Group: iGEM15_Hong_Kong-CUHK (2015-09-15)
Revision as of 12:24, 19 September 2015 by Fatfaatlai (Talk | contribs)
GFP-nanobody
The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102].
Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.
L: DNA ladder. Lane 2: GFP-nanobody.
This Part were verified by double digestion (Fig. 2) and sequencing.
Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| n/a | GFP-nanobody |