Reporter

Part:BBa_K1676037

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-09)
Revision as of 04:02, 10 September 2015 by Registry (Talk | contribs)

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GFP Reporter with Mutant RBS 1AC

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The second base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


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Categories
Parameters
None