Generator
Part:BBa_K1758370
Designed by: Team Bielefeld-CeBiTec 2015 Group: iGEM15_Bielefeld-CeBiTec (2015-08-27)
BlcR, repressor of blcABC operon, with promoter and RBS
Usage and biology
BlcR, a gene of Agrobacterium tumefaciens, represses the blcABC-operon. The latter enables the bacterium to grow on γ-butyrolactone as carbon and energy source as depicted in the following figure(Pan et al. 2013).
The Blc-Operon and the catabolism of GBL in A. tumefaciens. GHB: γ-hydroxybutyrate; GBL: γ-butyrolactone; SSA: Succinic semialdehyde; SA: Succinate.
Characterization – BlcR function
We performed EMSA-shifts and verified: BlcR binds to the operator site described in Pan et al. 2013
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 812 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 520
Illegal NgoMIV site found at 761 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |