Composite

Part:BBa_K1758344

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)
Revision as of 19:31, 18 September 2015 by Luzia (Talk | contribs) (→‎Usage and Biology)

Mercury responsive promoter with T7-promoter and UTR-sfGFP

mercury induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection

Usage and Biology

For the in vitro characterization we used a cell extract out of cells which contain the Plasmid ( BBa_K1758340). In addition to that we added Plasmid-DNA of the copper specific promoter merT with 5’UTR-sfGFP under the control of T7-promoter ( BBa_K1758344)to the cell extract. The T7-promoter is needed to get a better fluorescence expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 145


[edit]
Categories
//function/sensor/metal
Parameters
None