Composite

Part:BBa_K1648006

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-09-16)
Revision as of 03:01, 19 September 2015 by Fatfaatlai (Talk | contribs)

Insertion kit with GFP-nanobody

Consist of Insertion Kit(K1648004) and GFP-nanobody (K1648005) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications.

GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the characterization of level of expression of fused protein in Insertion Kit(K1648004) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aInsertion kit with GFP-nanobody