Composite

Part:BBa_K1648002

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-16)
Revision as of 01:57, 19 September 2015 by S1155046866 (Talk | contribs)

Construct Map for K1648002

Recombination Template for mamAB Operon with Promotor and Terminator

Consist of a template for recombination of mamAB operon(K1648000), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.

This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.

This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii. K16480001GEL.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal AgeI site found at 890
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 616


[edit]
Categories
Parameters
n/aRecombination Template for mamAB Operon with Promotor and Terminator