Composite

Part:BBa_K1813032

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-08-17)
Revision as of 08:43, 18 September 2015 by Rc (Talk | contribs)

LacIR, AgCPR and CYP2D6 Regulon

A construct used to achieve the simultaneous bacterial expression of HUMCYPDB1 (BBa_K1813005) and the A. gambiae NADPH p450 reductase (BBa_K1813010) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 3978
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4568
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.

[edit]
Categories
Parameters
n/aLacIR AgCPR CYP2D6