DNA

Part:BBa_K1732016

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)
Revision as of 15:25, 18 September 2015 by Donnalee (Talk | contribs)

pSB1C3-J23115-B0034-T7RNAP-sites-B0034-YFP

A control used for the estrogen biosensor which has restriction sites in place of the ER-LBD (BBa_K1732023). The sites added the amino acids ACLKLGGSTGGGSHNC between K179 and K180.

Estrogen control sites 2.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None