Composite

Part:BBa_K1813023

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-08-05)
Revision as of 05:33, 18 September 2015 by Mvanins (Talk | contribs)

nicF Expression Cassette with LacI Reversed

LacI Reversed nicF


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1853
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of LacI Reversed Ptac NicF Term

Part Description:


Part Origin

where part originated and Genus species more words if needed

Part Function

part functionstuff

Design and Acquisition

After synthesizing a codon-optimized NicF, we used standard assembly to created a composite part composed of NicF driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicF expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicF. All nicF constructs are contained within standard pSB1C3 vectors.

Experience

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Categories
Parameters
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