Part:BBa_K1813022
nicE Expression Cassette with LacI Reversed
LacI Reversed nicE
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2054
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1469
Background of Ptac NicE Term
Part Description:
Part Origin
where part originated and Genus species more words if needed
Part Function
part functionstuff
Design and Acquisition
After synthesizing a codon-optimized NicE, we used standard assembly to created a composite part composed of NicE driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicE expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicE. All nicE constructs are contained within standard pSB1C3 vectors.
Experience
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