Composite

Part:BBa_K1714006

Designed by: Hiroki ONO   Group: iGEM15_HokkaidoU_Japan   (2015-09-17)
Revision as of 23:54, 18 September 2015 by Kakurehana (Talk | contribs)

pBad/araC-RBS B0034-Ag43 thanatin 3mer as alpha domain-double terminator

Ag43 is a kind of outer membrane protein that is composed of some domains. First one is signal peptide that transport Ag43 to outer membrane. After transportation, the signal peptide is cleaved and left at inner membrane. Second one is alpha domain that has connection peptide to another Ag43 alpha domain. Last one is beta domain that has beta barrel structure to let the barrel there. Due to the function of a part of beta domain, called auto-chaperon domain, alpha domain is let outside of the membrane. This part has thanatin, a kind of antimicrobial peptide 3mer as alpha domain. This thanatin has Asp(Aspartic acid) as the last residue. So if thanatin domain is digested by AspN endoproteinase (NEB). By this enzyme, thanatin regain own toxicity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1612
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1894
    Illegal NgoMIV site found at 2404
    Illegal NgoMIV site found at 2425
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1413
    Illegal AgeI site found at 1485
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 2164
    Illegal AgeI site found at 2578
    Illegal AgeI site found at 2820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None