![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1758331:Design
Designed by: Team Bielefeld-CeBiTec 2015 Group: iGEM15_Bielefeld-CeBiTec (2015-08-30)
Revision as of 23:51, 18 September 2015 by Gila (Talk | contribs) (Created page with "<partinfo>BBa_K1758331SequenceAndFeatures</partinfo> ===Design Notes=== <html> We used the codon optimized, synthesized Sequence of RFP under the control of pbrA from iGEM Tea...")
No part name specified with partinfo tag.
Design Notes
We used the codon optimized, synthesized Sequence of RFP under the control of pbrA from iGEM Team Brown 2007(BBa_I721001 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA and the pSB1C3:
CGGCATCAGCACCTTGTC
CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA
cm_revTATACGCAAGGCGACAAG
TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
Source
designed after BBa_I721001 and BBa_K516030 synthesized by IDT
References
Sequence of E. coli by Synthesized by IDT