Part:BBa_K1807002
Escherichia coli Polyphosphate Kinase Enzyme
This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli.
Usage and Biology
PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
The E. coli PPK coding sequence was derived from the pBC29 plasmid, published by Akiyama et al., 1992. iGEM York 2015 obtained pBC29 with Dr Jay Keasling's kind assistance.
Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 396
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1750
- 1000COMPATIBLE WITH RFC[1000]
| None |