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Part:BBa_K1627005
Device to demethylate theobromine
This part contains genes that allow for the demethylation of theobromine. The part contains a synthetic operon consisting of ndmB, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of theobromine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of theobromine, a growth curve can be made that correlates culture growth with concentration of theobromine. Then if the strain is grown in a minimal media with an unknown concentration of theobromine, the growth can be compared to the growth curve to calculate the initial concentration of theobromine in the media.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 644
Illegal NheI site found at 839 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1595
Illegal BglII site found at 2091
Illegal BglII site found at 2562
Illegal XhoI site found at 3703 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4401
Illegal AgeI site found at 2230 - 1000COMPATIBLE WITH RFC[1000]
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