Composite

Part:BBa_K1688005

Designed by: Fredrik Lindeberg   Group: iGEM15_Uppsala   (2015-09-15)
Revision as of 21:13, 15 September 2015 by Registry (Talk | contribs)

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dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system)

BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal NgoMIV site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None