Composite
Part:BBa_K1688005
Designed by: Fredrik Lindeberg Group: iGEM15_Uppsala (2015-09-15)
dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system)
BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal NgoMIV site found at 2277 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |