Coding

Part:BBa_K1796007

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 22:02, 18 September 2015 by Nieyi (Talk | contribs)

nifB from Paenibacillus sp. WLY78

essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1156
    Illegal AgeI site found at 129
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1261


Parametre of Protein

Number of amino acids: 499

Molecular weight: 54869.8

Theoretical pI: 6.64

Amino acid composition: Ala (A) 46 9.2% Arg (R) 35 7.0% Asn (N) 17 3.4% Asp (D) 23 4.6% Cys (C) 16 3.2% Gln (Q) 19 3.8% Glu (E) 40 8.0% Gly (G) 42 8.4% His (H) 17 3.4% Ile (I) 30 6.0% Leu (L) 41 8.2% Lys (K) 25 5.0% Met (M) 11 2.2% Phe (F) 13 2.6% Pro (P) 25 5.0% Ser (S) 27 5.4% Thr (T) 17 3.4% Trp (W) 2 0.4% Tyr (Y) 13 2.6% Val (V) 40 8.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% 

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63 Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon C 2398 Hydrogen H 3849 Nitrogen N 701 Oxygen O 719 Sulfur S 27

Formula: C2398H3849N701O719S27 Total number of atoms: 7694

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 31370 Abs 0.1% (=1 g/l) 0.572, assuming all pairs of Cys residues form cystines


Ext. coefficient 30370 Abs 0.1% (=1 g/l) 0.553, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). 

                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.03 This classifies the protein as unstable.


Aliphatic index: 87.96

Grand average of hydropathicity (GRAVY): -0.253

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