Composite
J13002AvOp

Part:BBa_K1648048

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)
Revision as of 22:03, 18 September 2015 by Alyssaliu (Talk | contribs)

AvOprF induced by J13002

Construct Map for K1648048

OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. BBa_K1648048 provides constitutive transcription of oprF of Azotobacter vinelandii.

Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 555


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Categories
Parameters
n/aAvOprF induced by J13002