Coding

Part:BBa_K1850012:Design

Designed by: Lydia Goldberg   Group: iGEM15_Harvard_BioDesign   (2015-09-15)
Revision as of 21:54, 18 September 2015 by Lgoldberg (Talk | contribs)

fim


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 868
    Illegal AgeI site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected BBa_I13453, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.

fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ' fim subunits.



Source

The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.

References