Coding

Part:BBa_K1796014

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 15:02, 17 September 2015 by Registry (Talk | contribs)

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nifV promoted from Paenibacillus sp. WLY78

homocitrate synthase，provides the organic moiety in all three nitrogenase cofactors.

First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1110
    Illegal BamHI site found at 933
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
protein