Coding

Part:BBa_K1796013

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 14:56, 17 September 2015 by Registry (Talk | contribs)

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hesA promoted from Paenibacillus sp. WLY78

Function has not so far been determined , but Deletion analysis indicates that in addition to the core nif genes, hesA plays an important role in nitrogen fixation and is responsive to the availability of molybdenum. HesA a member of the ThiF-MoeB-HesA family and contains an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. The ThiF-MoeB-HesA family engage in an ATP-dependent process that activates the C-terminus of partner ubiquitin-like proteins by forming an acyl adenylate complex that facilitates sulfur transfer.

First, we promoted it ourselves(done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 143
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 143
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 143
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
protein