Regulatory

Part:BBa_K1652000:Design

Designed by: Martin Hanzel   Group: iGEM15_uOttawa   (2015-09-08)
Revision as of 18:29, 8 September 2015 by Registry (Talk | contribs)

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pPGK1Gx


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TSS in PGK1 is not well-defined, and there exists a region at the end of the promoter, about 100 bp long, that is very AT-rich. The promoter also contains two elements that resemble TATA boxes.


Source

S. cerevisiae

References