Protein_Domain

Part:BBa_K1729002

Designed by: Dani Bauhan and Richard Anthony   Group: iGEM15_RHIT   (2015-09-14)
Revision as of 20:29, 18 September 2015 by RicRHIT (Talk | contribs)

Yeast MRPS12 mitochondrial localization signal

This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of Saccharomyces cerevisiae mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview MRPS12]. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda et al.).

File:MRPS12 MLS2.jpg

Screen capture from SnapGene®

References

Branda, Steven S et al. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None