Generator

Part:BBa_K1758332

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)
Revision as of 23:02, 17 September 2015 by Luzia (Talk | contribs) (Created page with "PbrA-5-UTR-sfGFP <partinfo>BBa_K1758330 short</partinfo> Lead promoter with 5´untranslated reigion and sfGFP for detection ===Usage and Biology=== <html> For our biosensor w...")

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PbrA-5-UTR-sfGFP Lead repressor under control of constitutive promoter and strong RBS

Lead promoter with 5´untranslated reigion and sfGFP for detection


Usage and Biology

For our biosensor we use parts of the chromosomal lead operon of Cupriavidus metallidurans (Ralstonia metallidurans). The promoter that we use is PbrA. This part of the operon is regulated by the repressor pbrR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
    Illegal NheI site found at 488
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
Parameters
None