Coding

Part:BBa_K1813010

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)
Revision as of 05:45, 18 September 2015 by Rc (Talk | contribs)

A. gamb NADPH Reductase

The Anopheles gambiae NADPH - p450 reductase is a redox enzyme critical to the reuse of cytochrome p450 enzymes dependent on a supply of electrons for function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
    Illegal AgeI site found at 1453
    Illegal AgeI site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1969


Background

Transferring electrons from NADPH to a cytochrome p450 enzyme, this enzyme replenishes electrons used by a cytochrome p450 in the catalysis of a reaction ensuring the reusability of the enzyme. This coding sequence was synthesized and is codon optimized for E. coli. It was originally identified in Anopheles gambiae, the Anopheles Mosquito [2]. This part is also used in BBa_K1813018.

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Categories
Parameters
None