Composite

Part:BBa_K1651000

Designed by: YANG YUANZHAN   Group: iGEM15_BIT   (2015-09-13)
Revision as of 18:59, 18 September 2015 by Wzsnova (Talk | contribs)

PAI1 detector 

When the PAI1 spread into the amplifier, it will combine with a protein named lasR which is regulated by a constitutive promoter to form a complex. The PAI1-lasR complex will then activate the pLAS to produce a protein named rhlI.The rhlI will catalyze the production of PAI2, which will combine with another protein coming from the rhlIR circuit. And the rhlR is also regulated by a constitutive promoter like lasR. Now there comes to the PAI2-rhlR complex.

The PAI2-rhlR complex will directly activate the pRHL to enable the production of  super - folder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 2604
    Illegal NheI site found at 2627
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1773
    Illegal BamHI site found at 2901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3376
    Illegal BsaI.rc site found at 2513


[edit]
Categories
Parameters
None