Coding

Part:BBa_K1583111

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)
Revision as of 13:43, 4 September 2015 by Registry (Talk | contribs)

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pRha + CsgA_Mfp3_His fusion protein & pTET + RFP

The fusion protein of CsgA with Mfp3 and N-terminal His tag was improved by insertion of constitutively expressed RFP in a different operon to make the cells red fluorescent.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
Parameters
None