Reporter

Part:BBa_K567018

Designed by: Xiaopan Ma and Xiwen Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-30)
Revision as of 05:10, 30 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PT7-GFP-TAG-RFP

GFP and RFP linked with a flexible chain and a stop codon TAG is inserted in the flexible chain. This biobrick is under the control of T7 promoter and lac operator. This part is used to testify the function of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 191
    Illegal EcoRI site found at 1760
    Illegal XbaI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 191
    Illegal EcoRI site found at 1760
    Illegal NheI site found at 152
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 191
    Illegal EcoRI site found at 1760
    Illegal BamHI site found at 185
    Illegal XhoI site found at 1622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 191
    Illegal EcoRI site found at 1760
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 191
    Illegal EcoRI site found at 1760
    Illegal XbaI site found at 48
    Illegal AgeI site found at 1498
    Illegal AgeI site found at 1610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 840


[edit]
Categories
Parameters
chassisER2566
functionIPTG induced RFP and GFP expression
ligandsIsopropyl β-D-1-Thiogalactopyranoside (IPTG)