Regulatory

Part:BBa_K1728000

Designed by: Yung-Li Chen, Timothy Chan En Haw   Group: iGEM15_CGU_Taiwan   (2015-09-16)
Revision as of 18:26, 18 September 2015 by Allen (Talk | contribs)

IL8 toehold switch RNA sensor

IL8 toehold switch RNA sensor 

   Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site(RBS)in the loop. There  are three basic elements of toehold switches including 30bp trigger sequence, bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of oral cancer biomarker IL8(Interleukin 8) mRNA. Once the IL8 mRNA(IL8 partial sequence,BBa_K1728004)is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None