Coding

Part:BBa_K1179058

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)
Revision as of 18:02, 18 September 2015 by Phylars (Talk | contribs)

Cre entry vector

This part is submitted under the RFC 65 standard.

It encodes for Cre recombinase, which when present with LoxP sites performs Cre-Lox recombination and creates a Holliday junction.

Cre is one of the best understood Tyr-family recombinases. Although it is originally a integrase utilized by phages to infect its host. It is now one of the most commonly used tool for DNA knocking-out or insertion. If the two Lox sites are parallel, a deletion of sequence between them will happen. However, if the two loxP are anti-parallel, the sequence will be inverted into up-side-down. Such kind of activity is called invertase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None