Coding

Part:BBa_K1602010

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-14)
Revision as of 17:39, 18 September 2015 by Lenuchka (Talk | contribs)

D-Xylonolactone lactonase - xylC

xylC is coding for a D-xylonolactone lactonase from Caulobacter crescentus. It catalyzes the reaction of D-xylonolactone to D-xylonic acid. The sequence is codon optimized for the usage in E.coli.
https://static.igem.org/mediawiki/parts/4/47/BBa_K1602010_image_2.png

Characteristics


Molecular Weight 31585.59
Residues 289
Charge -9.0
Isoelectric Point 4.7584
A280 Molar Extinction Coefficients 44920 (reduced) 45170 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//awards/part_collection/2015
Parameters
None