Composite

Part:BBa_K1819004

Designed by: Lais Ribovski   Group: iGEM15_Brasil-USP   (2015-09-16)
Revision as of 01:59, 17 September 2015 by Laisribovski (Talk | contribs)

GFP+TetR+pTetR+mRFP1

This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2211
    Illegal AgeI site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None