Composite

Part:BBa_K1610105

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)
Revision as of 07:15, 6 September 2015 by Registry (Talk | contribs)

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pTS + RBS + yebF + ACT3m + Term

This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a motor protein sequence yebF (Bba_K1610300), a coding sequence (Bba_K1610201), and a terminator (Bba_B0015). This part is used to produce and secrete a modified human antichymotrypsin, a human extracellular protein that has been given the ability to bind and inhibit Granzyme B. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
    Illegal BglII site found at 1913
    Illegal XhoI site found at 2288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2214


[edit]
Categories
//awards/composite_part/nominee
Parameters
None