Coding

Part:BBa_K1583102:Design

Designed by: Stefan Robert Marsden, Hector Sanguesa Ferrer   Group: iGEM15_TU_Delft   (2015-09-08)
Revision as of 14:09, 18 September 2015 by Maxvthof (Talk | contribs) (Design considerations added)

pRha + CsgA + Hydroxyapatite-affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag.


Source

This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from (<a href="http://www.ncbi.nlm.nih.gov/gene/949055"target="_blank">E. coli K-12 MG1655</a>). The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations).

References