Part:BBa_K1157006:Experience

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Applications of BBa_K1157006

We used flow cytometry to test the individual fluorescence expression of our biobrick from 0 to 4 hours and in different concentration(10^-3, 10^-5,10^-7).

User Reviews

UNIQ4a94a34fb9d5c7ea-partinfo-00000000-QINU

• iGEM_Stockholm_2015

Method: Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 E. coli using the transformation protocol and were supposed to be assembled using the 3A assembly protocol. Results: During the initial lab weeks there were some trouble with transformation, later addressed to not using KCM buffer. The BBa_K082035 was ordered from IDT to speed up the process after repeated failed transformations and transformation troubleshooting were done in parallel. Conclusion: BBa_K082035 was ordered instead of cloned.

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•• iGEM_Stockholm_2015

Method: E. coli transformed with BBa_K1157006 were grown together with bacteria transformed with BBa_K082035 on normal agar plates without antibiotics. Results: The plates showed a slight red color change showed in Figure below. Figure: Plate showing E. coli with BBa_K082035 spread in horizontal streaks E. coli with BBa_1157006 spread as an M on top. A slight red color change is seen, as expected. Conclusion: Even though the two plates showed the correct color change, the results were however not reproducible at a later stage.

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