Translational_Unit
Part:BBa_K1587002
Designed by: Alexandre Le Scornet Group: iGEM15_Toulouse (2015-09-13)
pcyA with RBS
Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).
pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.
Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009) Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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