Intermediate

Part:BBa_K1789011

Designed by: Jiaqi Sun   Group: iGEM15_NUDT_CHINA   (2015-09-14)
Revision as of 14:45, 18 September 2015 by Sunjiaqi13 (Talk | contribs) (→‎Enzyme digestion test)

IAAH+Ter

It is the combination of IAAH and terminatior.

Usage and Biology

This part is constructed with IaaH and a terminatior.

IaaH is the tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. IaaH is the indoleacetimide hydrolase. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This pathway is relevantly easy to be expressed in prokaryotic cells, and its substrate (Trp) can be synthesized by host cells.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1005
  • 1000
    COMPATIBLE WITH RFC[1000]

Experimental Validation

Sequencing

This part is sequenced as correct after construction.

Enzyme digestion test

A double-enzyme digestion test was implemented to verify this part.

Iaah+ter酶切.jpg

The length of the digested DNA fits the design.

Functional Test

The function of this part can be verified with in the experimental validation of BBa_K1789022.

This parts functional test can be involved into k1789022.

[edit]
Categories
Parameters
None