RNA

Part:BBa_K1633005:Design

Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei   Group: iGEM15_NJU-China   (2015-09-13)
Revision as of 12:01, 18 September 2015 by ChenXi (Talk | contribs) (→‎Design Notes)


MOR siRNA-3 (siRNA for mouse Mu opioid receptor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.

This part is an artificially designed RNA strand. It serves as an element of the Team NJU CHINA RNAi module. We use them as siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we order them through a DNA synthesis company. We got four such RNA. MOR siRNA-3 is a backup.

NJU-China-parts-parts3.png

Figure 13. The sequence of MOR siRNA-3.

Source

We designed the MOR siRNA-3 and ordered the part from a DNA synthesis company.

References