RNA

Part:BBa_K1633003:Experience

Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei   Group: iGEM15_NJU-China   (2015-09-13)
Revision as of 11:28, 18 September 2015 by ChenXi (Talk | contribs) (→‎Applications of BBa_K1633003)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1633003

Usage and Biology

This part is a shRNA designed to target and degrade MOR mRNA. When this shRNA sequence is cut by restriction enzyme and then integrated into mammalian vector, this shRNA can play a RNAi function in mammalian cell lines. When the shRNA vector of MOR is transfected into mammalian cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.

CHARACTERIZATION

Interference efficiency of MOR siRNA-1 plasmid

To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.

NJU-China-parts-fig 2.png

Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids.

User Reviews

UNIQ35297ba17e2bbec7-partinfo-00000000-QINU UNIQ35297ba17e2bbec7-partinfo-00000001-QINU