Coding

Part:BBa_K1583102:Design

Designed by: Stefan Robert Marsden, Hector Sanguesa Ferrer   Group: iGEM15_TU_Delft   (2015-09-08)
Revision as of 10:51, 8 September 2015 by Registry (Talk | contribs)

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pRha + CsgA + Hydroxyapatite-affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag.


Source

The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).

References