Device

Part:BBa_K1797001

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 15:31, 17 September 2015 by Registry (Talk | contribs)

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Red Light Sensing System

This part is mainly composed of Cph8(BBa_K592018), ho1(BBa_I15008) and PcyA(BBa_I15009), which form the red light sensing system in E.coli. Cph8 is a synthetic red light sensor, consisting of Cph1, a natural red light sensor, and EnvZ, a histidine kinase. The most important part of the light sensor is PCB(phycocyanobilin), without which the red light sensor cannot work. However, PCB is not generated by E.coli naturally. So we need to introduce ho1 and PcyA into E.coli. These two enzymes can change heme into PCB. Here we combine the three main parts together to facilitate parts users.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3871
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3424
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None