Plasmid_Backbone

Part:BBa_K1680026

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-18)
Revision as of 07:54, 18 September 2015 by Registry (Talk | contribs)

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pETUE

Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 3053
    Illegal NgoMIV site found at 3213
    Illegal AgeI site found at 3270
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 374
    Illegal BsaI.rc site found at 1477


[edit]
Categories
//chassis/prokaryote/ecoli
//plasmid
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
Parameters
None