RNA

Part:BBa_K1797005

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 17:57, 17 September 2015 by Registry (Talk | contribs)

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sgRNA

This part includes sgRNA, which can be used to do site-specific gene editing when CRISPR Cas9 system is present. Users can use BspQI to digest the part and ligate with a DNA sequence that is targeted at a certain gene locus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 98
    Illegal BamHI site found at 650
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 666
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 369
    Illegal BsaI site found at 547
    Illegal SapI site found at 191
    Illegal SapI.rc site found at 180


[edit]
Categories
Parameters
None