Part:BBa_K1789022

IAA(+/+)S2

This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.

Usage and Biology

Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.

In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2724
  Illegal BamHI site found at 2311
  Illegal BamHI site found at 3669
  Illegal BamHI site found at 6072
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2431
  Illegal NgoMIV site found at 7088
  Illegal AgeI site found at 7576
  Illegal AgeI site found at 7606
  Illegal AgeI site found at 7636
  Illegal AgeI site found at 7666
  Illegal AgeI site found at 7696
  Illegal AgeI site found at 7726
  Illegal AgeI site found at 7756
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1304
  Illegal BsaI.rc site found at 5166
  Illegal BsaI.rc site found at 5370