Part:BBa_K1633003:Design
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.
Introduction
This part is an artificially designed RNA strand. It serves as an element of the Team NJU-CHINA RNAi module. We use it as the siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we synthesize the shRNA sequences with the help of a DNA synthesis company. We totally got four such shRNA plasmids.Although MOR siRNA-1 plasmid can efficiently knock down MOR expression, it does not show best interference efficiency and therefore serve as a backup.
Figure 1. The sequence of MOR siRNA-1.
Usage and Biology
This part is a shRNA designed to target and degrade MOR mRNA. When this shRNA sequence is cut by restriction enzyme and then integrated into mammalian vector, this shRNA can play a RNAi function in mammalian cell lines. When the shRNA vector of MOR is transfected into mammalian cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.
Source
We designed the MOR siRNA-1 sequence and ordered the sequence from a DNA synthesis company.