Part:BBa_K1789006
SCAF2
This part is a highly repetitive DNA sequence which can bind TALE protein specifically.
Usage and Biology
As is mentioned in our project description, different TALEs share a similar domain structure that enables them to bind the genome of the host cell and act as transcriptional effectors. By engineering those structures, we can build proteins that can bind with any DNA sequence that we desire.
In our project, we designed two different DNA binding motifs (DNA BMs). The sequences were chosen from Danio rerio CD154 gene in order to avoid homology with E.coli genome. Those BMs are sequenced as
BM1: 5’-GGAGGCACCGGTGG-3’
MB2: 5’-GATAAACACCTTTC-3’
Those sequences were repeated for more than 10 times in a plasmid, with different length of intervening sequence.
This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 8
Illegal AgeI site found at 50
Illegal AgeI site found at 92
Illegal AgeI site found at 134
Illegal AgeI site found at 176
Illegal AgeI site found at 218
Illegal AgeI site found at 260 - 1000COMPATIBLE WITH RFC[1000]
| None |