Coding
HR-His

Part:BBa_K1642001:Design

Designed by: Can Yin   Group: iGEM15_SJTU-BioX-Shanghai   (2015-08-15)
Revision as of 12:21, 9 September 2015 by EvaJiang (Talk | contribs)

Codon-optimized Halorhodopsin with His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
    Illegal NgoMIV site found at 292
    Illegal NgoMIV site found at 613
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

restriction


Source

Synthesized

References

[1]Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.

[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.